Composite

Part:BBa_K2583001:Design

Designed by: LiuJie   Group: iGEM18_Tsinghua-A   (2018-10-03)


ADH1_promoter-HRH4-mcherry-ADH1_terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830
    Illegal SapI site found at 2307


Design Notes

This is a composite part including HRH4 protein coding region encoded in part BBa_K2583000


Source

Uses a constitutive yeast promoter ADH1_promoter(BBa_K165015), HRH4 coding region(BBa_K2583000),mcherry(BBa_J06504), a yeast terminator ADH1_terminator(BBa_J63002).

References