Composite
Part:BBa_K2583001:Design
Designed by: LiuJie Group: iGEM18_Tsinghua-A (2018-10-03)
ADH1_promoter-HRH4-mcherry-ADH1_terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2157
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 180
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 830
Illegal SapI site found at 2307
Design Notes
This is a composite part including HRH4 protein coding region encoded in part BBa_K2583000
Source
Uses a constitutive yeast promoter ADH1_promoter(BBa_K165015), HRH4 coding region(BBa_K2583000),mcherry(BBa_J06504), a yeast terminator ADH1_terminator(BBa_J63002).